The principle and detailed analysis method by DLS measurement will be described in this section. Due to its nature, other equipment must be used to obtain detailed information such as the shape. It should also be noted that the obtained size includes a solvated layer around the particles. However, data analysis of DLS is based on the assumption that the particles are uniform and spherical. Understanding the Rayleigh Scattering Equation. Since the size information of particles, molecular aggregates, and emulsion droplet is extremely important, DLS is widely preferred by many colloid researchers. Dynamic light scattering (DLS) uses the above principle and provides size information of a wide range of particles easily and accurately. Thomson scattering is the elastic scattering of electromagnetic radiation by a free charged particle, as described by classical electromagnetism.It is the low-energy limit of Compton scattering: the particles kinetic energy and photon frequency do not change as a result of the scattering. By obtaining the autocorrelation function of the observed fluctuation, the particle size and distribution can be obtained. Light scattering intensity obtained from Mie theory is described by the following equation: Figure 1.12. Figure 1.12 represents schematically the principle of light laser scattering. Since this Brownian motion depends on the velocity of the particle size, the size can be estimated by observing the fluctuation corresponding to this velocity. Mie theory is an analytical solution of Maxwells equations for the scattering of electromagnetic radiation by particles of any size. Particles dispersed in a solution are constantly moving in Brownian motion.
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